Assisted Reproductive Technologies
The IVF Process
Jones Institute for Reproductive Medicine
IVF, in vitro fertilization, was first performed successfully in the United States at the Jones Institute for Reproductive Medicine, Eastern Virginia Medical School. Our specialized IVF team includes reproductive endocrinologists, IVF nurse coordinators, IVF laboratory specialists, and andrologists. The combined efforts of these individuals are responsible for our superior pregnancy IVF success rates.
Reproductive Genomics Program
The Reproductive Genomics Program at the Jackson Laboratory is funded by a Program Project grant from the Institute of Child Health and Human Development PO1 HD42137. It is a collaborative effort among the laboratories of John Eppig, Mary Ann Handel, and John Schimenti. The goal is to use random chemical mutagenesis of the genome to produce mouse models of infertility. At least 800 families of mice will be screened for infertility mutations over the next 4 years in a strategy that will produce over 100 mutant models.
Procedures for In Vitro Production of Bovine Embryos
The procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed in other laboratories at the University of Wisconsin (Parrish et al., 1986), University of Guelph (Xu et al., 1992), and University of Missouri (Hernandez-Ledezma et al., 1993). These procedures as used by our group have been published previously (Edwards et al., 1997; Paula-Lopes et al., 1998). Keep in mind that the protocols described here are not fixed but rather constantly evolve as new developments take place. Therefore, practitioners of IVP will be well advised to experiment with the procedures used, especially after reading of improvements made by other laboratories. This protocol is organized by day of the protocol with d 0 being the day of fertilization.
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